Journal: Experimental Biology and Medicine

Article Title: Annona atemoya leaf extract ameliorates cognitive impairment in amyloid-β injected Alzheimer’s disease-like mouse model

doi: 10.1177/1535370219886269

Figure Lengend Snippet: Molecular mechanisms responsible for anti-AD effects of AAL extract. (a) HT22 cells were exposed to H2O2 in the absence or presence of AAL extract (50 μg/mL) for 6 h. Antibody microarray assay was performed using the phospho-specific antibody microarray slide (Full Moon BioSystems). For data acquisition, GenePix 4100A scanner (Axon Instrument, USA) was used. The normalization data were analyzed using Genowiz 4.0™ (Ocimum Biosolutions). The phosphorylation ratio was calculated and represented as fold changes of indicated phosphoproteins after H2O2 treatment normalized to total protein expression (upper panel). Total protein quantification is shown (lower panel). (b) HT22 cells were exposed to H2O2 with or without various concentrations of AAL extract (0, 12.5, 25, or 50 μg/mL) for 6 h. Cell lysates were prepared from HT22 cells and equal amounts of protein were subjected to Western blotting using anti-phospho-CaMK2 β/ν/δ, GRK2, EGFR, Myc, FER, caveolin-1, NFκB p65, and MLRN 2 antibodies to validate the Ab microarray. (c) Protein extracts were prepared from hippocampal tissues in an Aβ-induced AD mouse model. Vehicle or various concentrations of AAL extract (50, 100, or 200 mg/kg) were administered to Aβ mice for 23 days. Western blotting was performed for anti-phospho-EGFR and phospho-GRK2. The validity of the two phospho-antibodies was determined using pre-stained protein marker (Bio-Rad). GAPDH was used as an internal control. Shown blots are representative results from three independent experiments.

Article Snippet: Molecular mechanisms responsible for anti-AD effects of AAL extract. (a) HT22 cells were exposed to H 2 O 2 in the absence or presence of AAL extract (50 μg/mL) for 6 h. Antibody microarray assay was performed using the phospho-specific antibody microarray slide (Full Moon BioSystems).

Techniques: Microarray, Phospho-proteomics, Expressing, Western Blot, Staining, Marker, Control

Journal: ACS Omega

Article Title: Increased Retention of Cardiac Cells to a Glass Substrate through Streptavidin–Biotin Affinity

doi: 10.1021/acsomega.1c02003

Figure Lengend Snippet: Functionalization of a glass substrate to increase cell retention. (A) Epoxy coating of a glass substrate followed by streptavidin conjugation and binding of biotinylated cells. (B) Fluorescent imagining of SA-Cy3 coating on epoxy slides and negative control/unmodified slides. Streptavidin density determined using the Cy3 calibration slide (scale bar = 1 mm).

Article Snippet: A Cy3 calibration slide (Full Moon Biosystems) was used to determine the average fluorophore density for each sample ( Figure S1 ).

Techniques: Conjugation Assay, Binding Assay, Negative Control

Journal: Current Neuropharmacology

Article Title: Gene Expression Profiling in Rodent Models for Schizophrenia

doi: 10.2174/157015910793358132

Figure Lengend Snippet: An Overview of Schizophrenia Animal Models Used in Microarray Studies

Article Snippet: Sprague–Dawley rats injected i.p. daily with Haloperidol or Risperidone and sacrificed 96 hours (acute treatment) or 4 weeks (chronic treatment) after initiation of drug treatment. , PXM oligo-nucleotide slides (Full Moon Biosystems) containing 8000 rat gene-specific, amino-modified oligonucleotides , Prefrontal/fronto-parieto-temporal cortices: Haloperidol:- acute: 28 genes - chronic: 9 genes Risperidone: - acute: 89 genes - chronic: 17 genes , [ ] .

Techniques: Microarray, Expressing, Infection, Virus, Clone Assay, Control, Isolation, Injection, Sequencing, Generated